epithelial cell medium kit  (Innoprot Inc)

Journal: bioRxiv

Article Title: An immune-poised state of human bronchial epithelial cells mediates RSV resistance in adults

doi: 10.64898/2026.02.02.703395

Figure Lengend Snippet: a , Dot plots for the 15 most significantly enriched hallmark pathways with age in ciliated cells in donor lungs of young children (N=13) and adults (N=12) by scRNA-seq dataset analyses. The status of age association of these 15 pathways in AT1 and AT2 cells, neonatal and adult BSCs (N=9 per age group) and day 21 ALI cultures (N=3 per age group) was also evaluated. b , Heatmap of the 47 age-associated inflammatory response genes in BSCs and ALI cultures. Each lane represents one donor. c , e , Representative Western blot assays of RIG-I, p-STAT3 Y705 , and STAT3 in neonatal and adult BSCs and ALI cultures. β-Actin was loading control. Each lane is one donor. d , f , Quantification of Western blot results by densitometry normalized to β-Actin. g , h , Representative antibody staining for RIG-I (fluorescence) and p-STAT3 Y705 (chromogenic) in infant and adult lungs (N=3 donors per age). Cells residing in lung mesenchyme in the same images showed comparable levels of staining for RIG-I and p-STAT3 Y705 between the two ages. Nuclei were counter stained by Hoechst dye in g and hematoxylin in h . Data were quantified by mean fluorescence intensity (MFI) for RIG-I staining and the percentage of bronchial epithelial cells positive for p-STAT3 Y705 . Panels on the right show enlarged outlined areas in panels on the left. The yellow dotted line marks basement membrane. *p<0.05 and ns, not significant by Student’s t-test (two-tailed).

Article Snippet: BSCs in TA samples were cultured and expanded in Small Airway Epithelial Cell Growth Medium (SAGM, PromoCell, Cat# C-21070) in the presence of SMAD/ROCK/mTOR inhibitors – .

Techniques: Western Blot, Control, Staining, Fluorescence, Membrane, Two Tailed Test

Journal: bioRxiv

Article Title: An immune-poised state of human bronchial epithelial cells mediates RSV resistance in adults

doi: 10.64898/2026.02.02.703395

Figure Lengend Snippet: a , Schema of mouse flu infection and scRNA-seq in a public dataset (GSE262927). b , Dot plots of enriched hallmark pathways in BEpiC and AT1+AT2 cells at each time point after infection. Mock group is baseline control. c , Schema of mouse flu infection for lung section staining. d , f , h , J , Representative images of fluorescence staining for H3K27me3, H3K27ac and RIG-I and chromogenic staining for p-STAT3 Y705 in mock and H1N1-infected mouse lungs. Cells residing in the lung mesenchyme within the same images, which showed comparable staining between mock and IAV infection, were control for BEpiC-specific changes following infection. The bottom panels show enlarged outlined areas in the upper panels. The dotted line marks basement membrane. Nuclei were stained by Hoechst dye in fluorescence staining and by hematoxylin in chromogenic staining. Scale bars, 50 µm. e , g , i , k , Quantification by MFI or the percentage of positive epithelial cells. Bar graphs show mean ± SEM. Each dot represents one mouse. *p<0.05 and ns, not significant by one-way ANOVA followed Tukey’s multiple comparison tests.

Article Snippet: BSCs in TA samples were cultured and expanded in Small Airway Epithelial Cell Growth Medium (SAGM, PromoCell, Cat# C-21070) in the presence of SMAD/ROCK/mTOR inhibitors – .

Techniques: Infection, Control, Staining, Fluorescence, Membrane, Comparison

Journal: bioRxiv

Article Title: Chronic Exposure to Nanoplastics Alters Stem Cell Type-Specific Mechanisms, Promoting Cancer Development

doi: 10.64898/2026.01.21.700811

Figure Lengend Snippet: Representative confocal images of fluorescent PS nanoplastic internalization after the indicated time in: ( A ) primary bone marrow CD34+ sorted hematopoietic immature cells or ( B ) epithelial or fibroblast cells isolated from primary mammary gland obtained from healthy donors or ( C ) MCF10A-derived models representing non-transformed mammary stem cells (MCF10A-CT), early (MC26) and more transformed (M1B26) cells as previously described . Nuclei are stained in blue, actin filaments in red, PS in green. ( D ) Confocal images of PS and PET nanoplastic internalization in MCF10A after 24 h compared to non-exposed controls. Nuclei are stained blue actin filaments red, PS green, PET orange. ( E ) Schematic diagram of the nanoplastic (NPLs) long-term exposure approach and analysis of their impact. ( F ) Quantification of the sphere-forming ability of untreated (CT) MCF10A cells or following 20 weeks of exposure to PS or PET nanoplastics (NPLs), n=5. ( G ) Quantification of total stem cell-derived progenitors by E-CFC assay , data represent the total number of colonies per 250 seeded cells, n=5. ( F , G , ) Each individual datum is represented. One way ANOVA is indicated on the graph by the P values. ( H ) Representation of the mean proportion of myoepithelial, mixed and luminal colonies quantified by E-CFC assay (n = 5). Data are expressed as the percentage of each subtype with a total number of scored colonies representing 100%. ( I ) Confocal images of acini. Nuclei are stained in blue, actin filaments in red, white arrows point to acini with a disorganized structure. Measurement of acini ( J ) solidity or ( K ) Ferret diameter ratio was quantified by image analysis using Fiji imaging software, n=109 to 131 individual acini. ( J , K ) Each individual data is represented. Mann-Whitney test is indicated on the graph by the P values.

Article Snippet: Cells were seeded at a density of 1500 cells/mL on 96-well ultra-low-attachment plates (Corning) in serum free basal mammary epithelial medium (Promocell) supplemented with B27, 20 ng/mL epithelial growth factor (EGF), 20 ng/mL basic fibroblast growth factor (bFGF), and 4 μg/mL heparin.

Techniques: Isolation, Derivative Assay, Transformation Assay, Staining, Imaging, Software, MANN-WHITNEY